UCI Chem 128 Introduction to Chemical Biology (Winter 2013)
Lec 07. Introduction to Chemical Biology -- DNA, RNA, and Cancer
View the complete course: http://ocw.uci.edu/courses/chem_128_introduction_to_chemical_biology.html
Instructor: Gregory Weiss, Ph.D.
License: Creative Commons BY-NC-SA
Terms of Use: http://ocw.uci.edu/info.
More courses at http://ocw.uci.edu
Description: This video is part of a 18-lecture undergraduate-level course titled "Introduction to Chemical Biology" taught at UC Irvine by Professor Gregory Weiss. Introduction to the basic principles of chemical biology: structures and reactivity; chemical mechanisms of enzyme catalysis; chemistry of signaling, biosynthesis, and metabolic pathways.
Introduction to Chemical Biology (Chem 128) is part of OpenChem: http://ocw.uci.edu/collections/open_chemistry.html
Recorded January 29, 2013.
Index of Topics:
Announcements
0:00:09- Week 3 Overview
0:01:28- Office Hours
0:02:51- Midterm 1 Info
0:06:24- Announcements
Lecture
0:06:40- Our Story Thus Far
0:09:48- Cutting and Pasting DNA
0:12:22- Restriction Enzymes Cut DNA Specifically
0:15:00- "Sticky Ends" Left Over from Restriction Enzymes for Pasting in New DNA
0:16:29- Cutting and Pasting DNA
0:18:42- Protein Modification by PCR
0:23:15- UV from Sunlight Cross-Links Thymines
0:25:09- E. Coli Photolyase
0:26:36- Protecting Your Cells By Sun Screen
0:28:39- Psoralens: The Scourge of Margaritaville
0:30:58- Psoralen Cross-Linked DNA
0:32:16- Margarita Photodermatitis (image)
0:33:06- Margarita Photodermatitis
0:33:24- Cells Must Advance or Die
0:34:57- DNA as a Big Nucleophile
0:38:26- Case Study: Omaha, Nebraska-Sept. 10, 1978
0:39:51- N-nitrosoamines = Potent DNA Alkylating Agents
0:41:13- The First Human Trials of N,N-Dimethylnitorsoamine
0:42:40- Bringing Home the Bacon
0:43:37- Why Bacon's (Mostly) Ok
0:45:00- Known Carcinogens
0:47:44- Paradox: Cause and Cure??
0:50:42- Excision Repair in Humans
0:52:34- Monofunctional Alkylating Agents: Often Readily Repairable
0:54:23- Bifunctional Cross-Linking Agent
0:54:58- Caution! Bifunctional DNA Alkylators
0:55:47- Excision Repair In Humans
0:56:46- Missing Or Deficient DNA Repair Proteins
0:57:25- Hundreds of Mutations Required to Cause Cancer
1:00:39- The p53 Tumor Suppressor Protein
1:02:05- Mutations to p53 are Bad News
1:02:52- p53 Suppressors Tumors
1:03:57- Sir Percival Pott
1:05:19- Testicular Cancer
1:06:13- Testicular Cancer, Slide 2
1:06:56- P450 Substrates as Potent DNA Alkylators
1:08:24- Benzopyrene: Pro-Epoxide DNA Alkylators
1:10:18- Toxins from Molds Growing on Grains
1:11:36- Nitrogen and Sulfur Mustards
1:13:35- The Re-Evaluation of RNA's Importance
1:14:41- What Levene Got Right
1:15:07- The Re-Evaluation of RNA's Importance
1:15:53- RNA: Soldier, Sailor, Tinker, Spy
Required attribution: Weiss, Gregory Introduction to Chemical Biology 128 (UCI OpenCourseWare: University of California, Irvine), http://ocw.uci.edu/courses/chem_128_introduction_to_chemical_biology.html. [Access date]. License: Creative Commons Attribution-ShareAlike 3.0 United States License (http://creativecommons.org/licenses/by-sa/3.0/us/deed.en_US).
