UCI Chem 128 Introduction to Chemical Biology (Winter 2013)
Lec 13. Introduction to Chemical Biology -- Protein Function and Enzymes
View the complete course: http://ocw.uci.edu/courses/chem_128_introduction_to_chemical_biology.html
Instructor: Gregory Weiss, Ph.D.
License: Creative Commons BY-NC-SA
Terms of Use: http://ocw.uci.edu/info.
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Description: This video is part of a 18-lecture undergraduate-level course titled "Introduction to Chemical Biology" taught at UC Irvine by Professor Gregory Weiss. Introduction to the basic principles of chemical biology: structures and reactivity; chemical mechanisms of enzyme catalysis; chemistry of signaling, biosynthesis, and metabolic pathways.
Introduction to Chemical Biology (Chem 128) is part of OpenChem: http://ocw.uci.edu/collections/open_chemistry.html
Recorded February 21, 2013.
Index of Topics:
0:00:29- Week 7 Overview
0:00:41- Our Story to Date...
0:03:20- Repeat Proteins
0:05:16- Equilibrium Constants to Describe the Strengths of Non-Covalent Interactions
0:06:25- Enzymatic Catalysts = Catalytic Receptors
0:06:55- Enzymes Work By Stabilizing & Thus Lowering the Energy of Transition States
0:07:03- Michaelis Constant for Measuring Catalysis
0:10:53- Typical Km Values
0:12:47- Typical Kcat Values
0:15:30- The Perfect Enzyme
0:18:02- Systematic Examining Enzymes
0:21:29- Kinases: Phosphorylation of Ser/Thr or Tyr
0:22:41- Can Inhibit Enzymes by Substrate Mimicry
0:24:24- The Kinase Active Site
0:26:03- The Mechanism of Kinase Catalysis
0:28:06- Kinase Activity is Tightly Regulated
0:31:17- Phosphorylation to Activate Kinase
0:34:19- Why Study Single Molecules?
0:37:44- How to Follow Enzymatic Catalysis with Single Walled Carbon Nanotubes
0:41:34- Single Molecule Bioelectronics
0:42:29- A Single Enzyme in the Nanocircuit
0:44:24- Before and After Enzyme Attachment
0:46:29- Watching cAMP-Dependent Protein Kinase A
0:49:18- Further Generalization: Protein A Kinase
0:55:55- Lysozyme as a Model Enzyme for Glycoside Hydrolysis
0:57:37- Mechanism of Lysozyme (Example of a Hydrolase)
1:02:07- Comparing Single Molecule Signals: Bioelectronic vs. FRET
1:02:14- Watching a Single Enzyme-Electronically
1:02:42- Lysozyme Exhibits Two Types of Binary Switching
1:04:23- Individual Lysozymes on Nanotubes Confirms Previous Studies
1:04:29- Lysozyme Processively Transits Through Peptide Cross-Links in the Substrate
1:05:29- Lysozyme Processively...Slide 2
1:08:22- Proteases Cleave Amide Bonds
1:09:19- Mutations In Protease-Based Pathways Can Have Dire Consequences
1:09:41- Apoptosis is Regulated By a Cascade of Protease Reactions
1:10:06- Cysteine-Based Proteases
1:10:46- Mechanism for Cysteine-Based Protease
1:12:21- Serine-Based Proteases
1:12:30- Serine Proteases
1:12:32- Zinc Proteases
1:12:37- Regulation of Proteases Through Pro-Enzymes
1:13:38- Inhibiting Protease Reversibility
1:14:35- Protein Based Inhibition of Proteases
Required attribution: Weiss, Gregory Introduction to Chemical Biology 128 (UCI OpenCourseWare: University of California, Irvine), http://ocw.uci.edu/courses/chem_128_introduction_to_chemical_biology.html. [Access date]. License: Creative Commons Attribution-ShareAlike 3.0 United States License (http://creativecommons.org/licenses/by-sa/3.0/us/deed.en_US).
